Reverse Transcription
First-strand cDNA.
Using in-house MMLV, we prepared first-strand cDNA. mRNA enrichment was achived using anchored oligo-dT primers. The resulting RNA:DNA hybrid becomes the template for (q)PCR and bulk RNA-Sequencing.
First-strand cDNA.
Using in-house MMLV, we prepared first-strand cDNA. mRNA enrichment was achived using anchored oligo-dT primers. The resulting RNA:DNA hybrid becomes the template for (q)PCR and bulk RNA-Sequencing.